We recently published an open-source and semi-automated pipeline that can be applied to the analysis of confocal immunofluorescence data. The program allows users to quantify subnuclear foci, as well as colocalized particles and pixel-based colocalization metrics (e.g. Pearson's Correlation Coefficient).

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This project was initiated by Sonny Panichnantakul, with additional support from Ayushi Patel and Elizabeth Tse.

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Click here to read more: https://doi.org/10.1016/j.dnarep.2021.103156

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Interested in using this pipeline?

* Click here to download the step-by-step instructions for MacOS.

* Click here to download the step-by-step instructions for Windows.

* Click here to download all of our programming scripts.

DISCLAIMER: the step-by-step instructions are subject to change; the documents are updated with the suggestions and feedback we receive from users.